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Dr R S Manik
Principal Scientist
Animal Biotechnology Centre NDRI,Karnal-132001 Tel: 01842259525
Current Research Area 
Ovum-Pick-Up/ In vitro production of embryos, Reproductive cloning, Embryonic stem cell, Female gamete cryopreservation etc.
  • Ph.D. (Livestock Production and Management)
  • M.Sc. (Livestock Production and Management)
Major Research Accomplishments

The technique of transvaginal ultrasonography for Ovum Pick Up was standardised and the feasibility of obtaining oocytes from cattle and buffaloes was demonstrated. Repeated collection of oocytes through OPU from cattle and buffalo showed no adverse effects on follicular growth and development.

Standardised the non-invasive technique of ultrasonography for monitoring follicular changes, pregnancy diagnosis, detection of ovulation and corpora lutea in normal cycling and superovulated cattle and buffaloes. Applying this technique the reasons for poor superovulatory responses, like inability of follicles to ovulate, shift, recruitment and passage of follicles from small to large size, predominance of two wave follicular activity, effect of presence of dominant follicle in cattle and buffalo, follicular population and dynamics in bovines were studied.

Standardised the protocols for superovulation both for indigenous and crossbred cattle  and many calves were produced from elite donors. A record number of ten calves were produced in one year using ET technology and the same has been recorded in Limica Book of Records.

Modified and simple technique of in vitro fertilization developed and results optimized in buffaloes using slaughterhouse ovaries. The optimization procedure involved series of experiments to test various types of sera, hormones, growth factors, different time intervals, culture and capacitation conditions for spermatozoa etc. Six calves were produced to reveal the feasibility of the technology.

Various steps involved in micromanipulation and cloning of embryos were standardised. Worlds first cloned calf was produced on 6th February, 2009 using hand guided cloning technique. Using the same technique, a calf GARIMA was produced on 6th June, 2009.
Putative buffalo embryonic stem cells were produced by culture of inner cell mass cells obtained from in vitro produced buffalo blastocysts and when subjected to induced differentiation by culture in the presence of all-trans retinoic acid, they differentiated to neuron-like cells, which were found to express NF-68 gene, a specific marker for neuronal cells confirming that the cells produced were indeed neurons.  

 A simple and rapid method was standardized for the cryopreservation of buffalo oocytes by vitrification. It was demonstrated that it was possible to cryopreserve buffalo oocytes by vitrification. However, protocols effective for the vitrification of cumulus-oocyte-complexes were not suitable for denuded oocytes

Animal Birth Control vaccine (TALSUR), which was developed at National Institute of Immunology, New Delhi was first time tested in buffalo and cattle bulls and bucks at NDRI and protocol of injection and testing method was developed. The technology developed was also tested in village Bhusli, near Karnal where the buffalo bulls were sterilised and this news was reported in NATURE.

Guidance to students:



  • The best poster award on the abstract entitled “Expression of transcription factors and in vitro differentiation of buffalo (Bubalus bubalis) embryonic stem cells cultured on different feeder layers” by the Indian Society for Buffalo Development, in International Buffalo Conference, held during February 1-4, 2010, at New Delhi.
  • AJAS Purina Outstanding Research Award (1996-99) based on best research article, given by ‘Asian-Australian Association of Animal Production Societies, Korea. The award carried a cash prize of US $ 500
  • Awarded Food and Agricultural Organization (FAO) of United Nation fellowship to USA (2000).
  • Prof. Nils Lagerlof Memorial Award for best paper on animal reproduction (1997). The award carried a medal and citation.
  • Awarded World Bank fellowship under NARP (1995).
  • Consulting editor for Journal of Maharashtra Agricultural Universities, in 2005.
  • Member editorial board for Indian Journal of Animal Production and Management from 1998 to 2000.
  • Awarded best poster award from Indian Society for Study in Reproduction and Fertility, Mumbai in 2006.
  • Fellow of National Academy of Dairy Science (India) 2014
  • National-5
  • International-2
Patents, Technology, Methodology, Genetic Stock, Variety, etc.:
  • Production of Cloned calves through hand guided cloning technique
  • Establishment of procedures for development of putative buffalo embryonic stem cells
  • Ovum Pick-Up technology both from cattle and buffalo
  • Transrectal ultrasonography for pregnancy diagnosis and for studying ovarian function
  • Embryo transfer technology
  • Production of calves through In vitro production of embryos from slaughter house ovaries
  • Sah, R.A., George, A., Singh, M.K., Kumar, D., Anand, T., Chauhan, M.S., Manik, R.S., Palta, P. and S.K. Singla (2009). Pregnancy established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis). Theriogenology, 71:1215-1219.
  • Gautam, S.K., Verma, V., Palta, P., Chauhan, M.S. and Manik, R.S. (2008). Effect of type of cryopretectant on morphology and developmental competence of in vitro matured buffalo (Bubals bubalis) oocytes subjected to slow freezing or vitrification. Reproduction Fertility and Development, 20: 490-496.
  • Verma., Gautam, S.K., Singh, B., Manik, R.S., Palta, P., Singla, S.K., Goswami, S.L. and Chauhan, M.S. (2007). Isolation and characterization of embryonic stem cell-like cells from in vitro produced buffalo (Bubalus bubalis) embryos. Molecular Reproduction and Development 74: 520-529.
  • Manik,., Singla, S.K. and Palta, P. (2003). Coolection of oocytes through transvaginal ultrasound-guided aspiration of foolicles in an Indian breed of cattle. Animal reproduction Science 76:155-161.
  • Manik., R.S., Palta, P., Singla, S.K. and Sharma, V. (2002). Folliculogenesis in buffalo (Bubalus bubalis): a review. Reproduction Fertility Development 14: 315-325.
  • Manik, R.S., Chauhan, M.S., Singla, S.K. and Palta, P. (2002). Transvaginal ultrasound-guided aspiration of follicles from Indian buffaloes (Bubalus bubalis) with reproductive problems. Veterinary Record 150: 22-24
  • Manik, R.S., Singla, S.K., Palta, P. and Madan, M.L. (1998). Effect of presence of a dominant follicle on the superovulatory response in buffalo (Bubalus bubalis). Theriogenology, 50: 841-852.
  • Palta, P., Kumar, M., Jailkhani, S., Manik, R.S. and Madan, M.L. (1997). Changes in peripheral inhibin levels and follicular development following treatment of buffalo with PMSG and Neutra PMSG for superovulation. Theriogenology. 48: 233 240.
  • Madan, M.L., Chauhan, M.S., Singla, S.K. and Manik, R.S. (1994). Pregnancies established from water buffalo (Bubalus bubalis) blastocyst derived from in vitro fertilized oocytes and co cultured with cumulus and oviductal cells. Theriogenology, 42: 591 60.
  • Singh, N.P., Manik, R.S. and Raina, V.S. (1989). Effect of cysteine fortification on preservability of buffalo semen in milk whey extenders. Theriogenology, 32: 979 986.
  • Selokar NL, Saini M, Palta P, Chauhan MS, Manik RS, Singla SK. (2014). Hope for Restoration of Dead Valuable Bulls through Cloning Using Donor Somatic Cells Isolated from Cryopreserved Semen. PLOS ONE 9(3): e90755.
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