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Dr. Anil Kumar Puniya
Principal Scientist
Principal Scientist and Head, DM Division ICAR-NDRI Karnal
Email:Anil.Puniya@icar.gov.in, akpuniya@gmail.com
  
Current Research Area 
Anaerobic Microbial Fermentation
 
Qualifications:
  • Ph.D. (Dairy Microbiology), 1994; National Dairy Research Institute, Karnal
  • M.Sc. (Microbiology), 1988; Gurukul Kangri University, Hardwar
  • B.Sc. (Botany, Chemistry and Zoology), 1985; Meerut University, Meerut
 
 
Major Research Accomplishments
 
Untitled Document
  • In our research group, we have reported two novel genera of anaerobic fungi (Oontomyces anksri & Buwchfawromyces eastonii), which were only the 7th & 8th genera since their discovery in mid-seventies.
  • Developed a method for differentiation of genera/ species of these fungi using D1/D2 domain of large-subunit r-DNA. Our research group also developed the formulation of most-efficient direct-fed microbials that improved animal nutrition & productivity.
  • Contributed towards understanding the diversity of methanogens & methane-mitigation interventions. Our research group also isolated health-promoting probiotics that produces conjugated linoleic/ linolenic acids, transform isoflavones or control postprandial hyperglycemia.
  • Our research group also developed technologies like 'methods for long-term preservation of anaerobic fungi'; 'micro-encapsulation of probiotics'; 'method for detection of antibiotics in milk'; and 'SSF-ENLAC technology'.
 
Awards:
  • Fellow of NAAS, 2020, National Academy of Agricultural Sciences
  • 'Associate Editor'; Board of Systems Microbiology (Specialty Section of "Frontiers in Microbiology")
  • APSI Award, 2019, Academy of Plant Sciences of India
  • Certificate of Appreciation, (ABRCMS, Anaheim, California), 2019, The Annual Bio-medical Research Conference for Minority Students’, USA
  • Best Oral Presentation Award (NPDF), 2019, 7th International Conference on Biological Sciences (9th World food & Agriculture Congress), New Delhi
  • Fellow of BRSI, 2019, Biotech Research Society of India
  • AFN-Membership; The Netherlands, 2019, Anaerobic Fungi Network
  • Elsevier, BIORESTEC ('Bioresource Technology'), 2018, 'Bioresource Technology' for Bioenergy, Bioproducts & Environmental Sustainability, Spain
  • Fellow of FMSc, 2018, Indian Academy of Microbiological Sciences
  • Fellow of FMSc, 2018, Indian Academy of Microbiological Sciences
  • Awarded Second Best Prize in E-Poster presentation, 2018, Processing & value addition towards efficiency; 46th Dairy Industry Conference, Kochi, Kerala
  • Best Poster Award, 2017, National Seminar on Food Processing India; GJUS&T, Hisar
  • Elsevier - Awarded Certificate of Recognition, 2016, ‘Small Ruminant Research’ and ‘Energy Conservation & Management’
  • Wiley - Awarded Reviewer Certificate, 2016, International Journal of Dairy Technology (Society of Dairy Technology)
  • Elsevier - Awarded Certificate of Recognition, 2015, Animal Feed Science & Technology
  • Elsevier - Awarded Certificate of Outstanding Contribution, 2015-16, Journal of Integrative Agriculture (Official Publication of Chinese Academy of Sciences)
 
 
Training:
  • Training programme on ‘Data Analysis using SAS’, August 1-6, 2011; NDRI, Karnal (sponsored by NAIP (ICAR), Govt of India)
  • ‘E-learning for Dairy Science Faculty’ at NAARM, Hyderabad, March 2-12, 2010; NAARM, Hyderabad (sponsored by NAIP (ICAR), Govt of India)
  • ‘Molecular identification and characterization of anaerobic fungi’; 4 weeks, 2007; IBERS, University of Wales, Aberystwyth, UK (Sponsored by INSA, New Delhi, and Royal Society, London)
  • ‘Sixth Foundation Training Programme for Scientists and Technologists’, November 20, 2006 - January 25, 2007, Indian Institute of Public Administration’, New Delhi (Sponsored by ‘DST’, Govt of India)
  • Workshop on ‘Computational biology and bioinformatics’ October 10-13, 2006 NDRI, Karnal (sponsored by DBT, Govt of India)
  • Workshop on ‘Exploration of Microbial Diversity: A Polyphasic Approach’ September 30 to October 12, 2002 Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh
  • Workshop-cum-training programme, ‘Molecular tagging of inoculant strains for ecological studies’ August 24-30, 2000, Indian Agricultural Research Institute, New Delhi (sponsored by NATP (ICAR), Govt of India)
  • Orientation course, 1999; Academic Staff College, Kurukshetra University, Kurukshetra
 
 
Patents, Technology, Methodology, Genetic Stock, Variety, etc.:
  • Discovery of novel genera and species of anaerobic fungi "Oontomyces anksri" from the digestive tract of Indian camel & "Buwchfawromyces eastonii" from buffalo faeces
  • Development of genetic marker for molecular phylogeny & rapid method for differentiation of anaerobic rumen fungi and PCR based method for identification of multiple genera & species
  • Methods for long-term preservation of anaerobic fungi
  • Development of SSF-ENLAC technology and formulation of direct-fed microbials
  • Probiotics for human health (CLA/ CLNA production; antidiabetic and anti-obesity effects)
  • Mitigation of enteric methane
 
 
Publications:
  • Dagar SS, Singh N, Goel N, Kumar S, Puniya AK 2014 Role of anaerobic fungi in wheat straw degradation and effects of plant feed additives on rumen fermentation parameters in vitro. Beneficial microbes, 1-8
  • Mandal S, Hati S, Puniya AK, Khamrui K, Singh K 2014 Enhancement of survival of alginate-encapsulated Lactobacillus casei NCDC 298. Journal of the Science of Food and Agriculture. DOI: 10.1002/jsfa.6514
  • Kumar S, Dagar SS, Hadi S, Ebrahimi, Malik RK, Upadhyay RC, Puniya AK 2014 Prospective Use of Bacteriocinogenic Pediococcus pentosaceus as Direct-Fed Microbial Having Methane Reducing Potential. Journal of Integrative Agriculture. Advance Online Publication Doi:10.1016/S2095-3119(14)60832-7
  • James L, Puniya AK, Singh K 2014 A comparative evaluation of the inulin utilization potential of two Bifidobacterium animalis subsp. lactis strains for the development of a synbiotic powder. International Journal of Biological Research 2(2), 121-124
  • Ahmed HA, Sirohi SK, Dagar SS, Puniya AK, Singh N 2014 Effect of Supplementation of Selenomonas ruminantium NDRI-PAPB 4 as Direct Fed Microbial on Rumen Microbial Population in Karan Fries Male Calves Indian Journal of Animal Nutrition 31(1), 20-26
  • Sathe G and Puniya AK 2014 Screening of ß-galactosidase positive probiotic dairy yeasts. Indian Journal of Dairy Scince, 67(4):
  • Sirohi SK, Chaudhary PP, Singh N, Singh D, Puniya AK 2013 The 16S rRNA and mcrA gene based comparative diversity of methanogens in cattle fed on high fibre based diet. Gene, 523 (2), 161-166
  • Kumar S, Dagar SS, Sirohi SK, Upadhyay RC, Puniya AK 2013 Microbial profiles, in vitro gas production and dry matter digestibility based on various ratios of roughage to concentrate. Annals of Microbiology, 63 (2), 541-545
  • Sirohi SK, Choudhury PK, Puniya AK, Singh D, Dagar SS, Singh N 2013 Ribosomal ITS1 sequence-based diversity analysis of anaerobic rumen fungi in cattle fed on high fiber diet. Annals of Microbiology, 63 (4), 1571-1577
  • Sirohi SK, Choudhury PK, Dagar SS, Puniya AK, Singh D 2013 Isolation, characterization and fibre degradation potential of anaerobic rumen fungi from cattle. Annals of Microbiology, 63 (3), 1187-1194
  • Kumar S, Dagar SS, Puniya AK, Upadhyay RC 2013 Changes in methane emission, rumen fermentation in response to diet and microbial interactions. Research in Veterinary Science, 94 (2), 263-268
  • Ahmed HA, Sirohi SK, Puniya AK, Sheel R 2013 Influence of addition of propionic acid producing bacteria on dry matter digestibility and rumen fermentation profiles in wheat straw containing total mixed ration in vitro. Indian Journal of Animal Nutrition 30 (3), 272-280
  • Sirohi SK, Dagar SS, Singh N, Chaudhary PP, Puniya AK, Singh D 2013 Differential rumen microbial dynamics and fermentation parameters in cattle fed on high fibre and high concentrate diets. Indian Journal of Animal Nutrition, 30 (1), 60-66
  • Renuka, Puniya, M., Sharma, A., Malik, R.K., Upadhyay, R.C., Puniya, A.K. (2013) Influence of pediocin and enterocinon in-vitro methane, gas production and digestibility. Intl J Current Microbiol Appl Sci, 2(11):132-142.
  • Mandal S, Hati S, Puniya AK, Singh R, Singh K 2013 Development of synbiotic milk chocolate using encapsulated Lactobacillus casei NCDC 298. Journal of Food Processing and Preservation, 37 (5), 1031-1037
  • Attri P, Singh J, Puniya AK, Dhanda S 2012 SDS-PAGE analysis of membrane and extracellular proteins of vancomycin resistant Pediococcus acidilactici. Annals of Biology, 28 (1), 9-11
  • Kumar S, Dagar SS, Puniya AK 2012 Isolation and characterization of methanogens from rumen of Murrah buffalo. Annals of Microbiology, 62 (1), 345-350
  • Nagpal R, Puniya AK, Sehgal JP, Singh K 2012 Survival of anaerobic fungus Caecomyces sp. in various preservation methods: a comparative study. Mycoscience, 53(6): 427–432
  • Goel G, Kumar A, Beniwal V, Raghav M, Puniya AK and Singh K 2011 Degradation of tannic acid and purification and characterization of tannase from Enterococcus faecalis. International Biodeterioration and Biodegradation, 65: 1061-1065
  • Nagpal R, Puniya AK, Sehgal JP, Singh K 2011 In vitro fibrolytic potential of anaerobic rumen fungi from ruminants and non-ruminant herbivores. Mycoscience, 52 (1), 31-38
  • Dagar SS, Kumar S, Mudgil P, Singh R, Puniya AK 2011 D1/D2 domain of large subunit rDNA for differentiation of Orpinomyces spp. Applied and Environmental Microbiology, 77 (18): 6722–6725
  • Shrivastava B, Thakur S, Khasa YP, Gupte A, Puniya AK, Kuhad RC 2011 White-rot fungal conversion of wheat straw to energy rich cattle feed. Biodegradation, 22: 823–831.
  • Das S, Kumar N, Raghu HV, Haldar L, Gaare M, Singh VK and Puniya AK 2011 Microbial based assay for specific detection of ß-lactam group of antibiotics in milk. Journal of Food Science and Technology, 123-132
  • Sirohi SK, Pandey N, Mohini M, Puniya AK, Kundu SS, Thube H 2011 Effect of monensin and anthraquinone supplementation on rumen fermentation and methane mitigation in vitro. Indian Journal of Animal Science, 81 (8), 859-863
  • Malashree L, Mudgil P, Dagar SS, Kumar S, Puniya AK 2011 ß-Glucosidase activity of lactobacilli for biotransformation of soy isoflavones. Food Biotechnology, 26:154–163
  • Mandal S, Puniya AK and Singh K (2011) Evaluation of probiotic properties of Lactobacillus casei. Beverage and Food World, 38: 57-59.
  • Nagpal, R., Puniya, A.K., Singh, K. Sehgal, J.P. (2010) Influence of bacteria and protozoa from the rumen of buffalo on in-vitro activities of anaerobic fungus Caecomyces sp isolated from the feces of elephant, J. Yeast Fungal Res, 1(8):152 – 156.
  • Saxena, S., Sehgal, J.P., Puniya, A.K., Singh, K. (2010) Effect of administration of rumen fungi on production performance of lactating buffaloes, Beneficial Microbes, 1(2): 183-188.
  • Mandal S, Hati S, Puniya AK, Singh K 2010 Evaluation of cell-surface-hydrophobicity, antimicrobial activity and prebiotic utilization of Lactobacillus spp. Indian Journal of Dairy Science 63 (3), 178
  • Mandal S, Hati S, Basu S, Puniya AK, Singh R, Singh K 2010 Effect of k-carrageenan concentrations on survival of microencapsulated Lactobacillus casei NCDC 298. Indian Journal of Dairy Science, 63 (6), 470-
  • Puniya AK, Reddy CS, Kumar S and Singh K 2009 Influence of sunflower oil on conjugated linoleic acid production by Lactobacillus acidophilus and Lactobacillus casei. Annals of Microbiology, 59: 505-507
  • Shelke SK, Chhabra A, Puniya AK and Sehgal JP 2009 In vitro degradation of sugarcane bagasse based ruminant rations using anaerobic fungi. Annals of Microbiology, 59: 415-418
  • Nagpal R, Puniya AK and Singh K 2009 In-vitro activities of immobilized anaerobic rumen fungus Caecomyces sp for its use as direct-fed microbials. Journal of Animal and Feed Science, 18:758–768
  • Sehgal JP, Jit D, Puniya AK and Singh K 2008 Influence of anaerobic fungal administration on growth, rumen fermentation and nutrient digestion in female buffalo calves. Journal of Animal and Feed Science, 17: 510–518
  • Puniya AK, Chaitanya S, Tyagi AK, De S and Singh K 2008 Conjugated linoleic acid producing potential of lactobacilli isolated from the rumen of cattle. Journal of Industrial Microbiology and Biotechnology, 35: 1223-1228
  • Tripathi VK, Sehgal JP, Puniya AK andSingh K 2007 Hydrolytic activities of anaerobic fungi from wild blue bull (Boselaphus tragocamelus), Anaerobe, 13: 36-39
  • Goel G, Puniya AK and Singh K 2007 Phenotypic characterization of tannin–protein complex degrading bacteria from faeces of goat. Small Ruminant Research, 69: 217–220.
  • Tripathi VK, Sehgal JP, Puniya AK and Singh K 2007 Effect of administration of anaerobic fungi isolated from cattle and wild blue bull (Boselaphus tragocamelus) on growth rate and fibre utilization in buffalo calves. Archives of Animal Nutrition, 61: 416-423
  • Dey A, Sehgal JP, Puniya AK Singh K 2007 Morphological and metabolic characteristics of rumen anaerobic fungus Orpinomyces species. Environment and Ecology. 25(2): 312-314.
  • Dayanand TL, Nagpal R, Puniya AK, Sehgal JP and Singh K 2007 In-vitro degradation of urea-NH3 treated wheat straw using anaerobic ruminal fungi. Journal of Animal and Feed Science, 16: 484-489
  • Thareja A, Puniya AK, Goel G, Nagpal R, Sehgal JP, Singh PK and Singh K 2006 In-vitro degradation of wheat straw by anaerobic fungi from small ruminants. Archives of Animal Nutrition, 60: 412-417
  • Mandal S, Puniya AK andSingh K 2006 Effect of alginate concentrations on survival of microencapsulated Lactobacillus casei NCDC-298. International Dairy Journal, 16: 1190-1195
  • Goel G, Puniya AK and Singh K 2005 A Study on Tannic acid resistance in ruminal streptococcal isolates. Journal of Basic Microbiology, 45: 243-245
  • Goel G, Puniya AK and Singh K 2005 Xylanolytic activity of ruminal Streptococcus bovis in presence of tannic acid. Annals of Microbiology, 4: 295-298
  • Parasher R, Malik RK and Puniya AK 2005 Extraction, purification and characterization of exopolysaccharide produced by Lactococcus lactis PM 23 using formulated Paneer whey. Asian Journal of Microbiology, Biotechnology and Environmental Science. 7: 13-17
  • Manikumar B, Puniya AK, Singh K and Sehgal JP 2004 In-vitro degradation of cell-wall and digestibility of cereal straws treated with ruminal anaerobic fungi. Indian Journal of Experimental Biology, 42: 636-638
  • Dey A, Sehgal JP, Puniya AK and Singh K 2004 Influence of anaerobic fungal culture (Orpinomyces sp) administration on growth rate, ruminal fermentation and nutrient digestion in calves. Asian Australasians Journal of Animal Sciences, 17: 820-824
  • Rane S, Puniya AK and Singh K 2004 Biochemical changes on solid state fermentation of sugarcane bagasse using fungal cultures and nitrogen-fixing bacteria. Animal Nutrition and Feed Technology, 4: 63-69
  • Rane S, Puniya AK and Singh K 2004 Effect of fungi and N-fixing bacteria on the solid state fermentation of rice straw. Indian Journal of Animal Science, 74: 211-215
  • Chopra P, Singh D, Verma V, Puniya AK2004 Bioremediation of melanoidin containing digested spent wash from cane molasses distillery with white rot fungus, Coriolus versicolor. Indian Journal of Microbiology. 44: 197-200
  • Mor S, Singh K and Puniya AK 2005 Biodetoxification of preformed aflatoxins in milk products. Asian Journal of Microbiology Biotechnology and Environmental Sciences, 7: 19-22.
  • Rane S, Puniya AK and Singh K 2003 Effect of hydrolytic crude enzymes on degradation of sugarcane bagasse and rice straw. Indian Journal of Animal Nutrition. 20: 109-113
  • Mor S, Puniya AK and Singh K 2003 Aflatoxin degradation by cell-free extracts of detoxifying organisms in broth. Indian Journal of Dairy Science. 56: 56-58
  • Rane S, Puniya AK and Singh K 2003 A pilot scale study on ensiling of crop-wastes using crude enzyme and lactic acid cultures. Indian Journal of Microbiology, 43: 115-118
  • Mor S, Puniya AK and Singh K 2002 Degradation of preformed aflatoxins by detoxifying organisms. Indian Journal of Animal Science, 73: 315-318
  • Manikumar B, Puniya AK and Singh K 2002 Effect of ruminal fungi on in vitro degradation of rice straw. Indian Journal of Animal Science, 73: 312-314
  • Manikumar B, Puniya AK and Singh K 2002 Influence of ruminal fungi on degradation of wheat straw in vitro. Indian Journal of Microbiology, 42: 133-136
  • Kumar R, Aneja KR, Puniya AK, Roy P, Sharma M, Gupta R and Shobharam 2001 Changes pattern of biotypes, phage types and drug resistance of Salmonella typhi in Ludhiana since 1980. Indian Journal of Medical Research, 113: 175-180
  • Puniya AK, Chhabra A and Prasad T 1998 A study on 3-hydroxy-4 (1H)-pyridone degrading potential of rumen microbial population. Indian Journal of Dairy and Bioscience, 9: 65-69
  • Puniya AK, Chhabra A and Prasad T 1996 Biotransformation of 3-hydroxy - 4 (1h) pyridone by the rumen microflora from different animal sources. International Biodeterioration and Biodegradation, 35: 41-43
  • Puniya AK, Shah KG, Hire SA, Ahire RN, Rathod HP and Mali RS 1996 Bioreactor for solid state fermentation of agro-industrial wastes. Indian Journal of Microbiology, 36: 177-178
  • Puniya AK and Singh K 1995 Biochemical changes during solid substrate fermentation of wheat straw. Indian Journal of Microbiology 35: 211-215
  • Puniya AK and Singh K 1995 Determination of enzyme activities of lignocellulolytic strains in solid substrate fermentation products. Indian Journal of Animal Nutrition, 12: 205-212
  • Zadrazil F &Puniya AK 1995 Studies on effect of particle size during solid state fermentation of sugarcane bagasse into animal feed using white rot fungi. Bioresource Technology, 54: 85-87
  • Puniya AK, Zadrazil F and Singh K 1994 Influence of gaseous phases on lignocellulose degradation by Phanerochaete chrysosporium. Bioresource Technology, 47 (1): 181-183
  • Zadrazil F and Puniya AK 1994 Influence of carbon dioxide on lignin degradation and digestibility of lignocellulosics treated with Pleurotus sajor-caju. International Biodeterioration and Biodegradation, 33: 237–244
  • Singh K, Puniya AK and Neelakantan S 1994 Biochemical profiles of ensiled wheat straw with rumen liquor and molasses. Indian Journal of Animal Science, 64 (1): 66-70
  • Singh K, Puniya AK and Neelakantan S 1994 Degradation of wheat straw components during two stage solid substrate fermentation with Coprinus fimetarius and Azotobacter chroococcum. Indian Journal of Dairy Science, 47 (4): 314-318
  • Zadrazil F, Puniya AK and Singh K 1993 Studies on effect of different gas compositions on degradation characteristics of crop-residues and resulting digestibility with Pleurotus sajor-caju. Indian Journal of Microbiology, 33(4): 249-252
  • Zadrazil F and Puniya AK 1993 Efficiency of straw upgrading using Pleurotus sajor-caju at high carbon dioxide and different oxygen concentrations. Journal of Applied Animal Research, 4 (2): 73-82
  • Puniya AK, Singh K and Zadrazil F 1992 Influence of gaseous metabolites during solid-state fermentation of lignocellulosics with Coprinus fimetarius. Indian Journal of Animal Nutrition 9(4), 228-231
  • Gruninger RJ, Puniya AK, Callaghan TM, Edwards, JE, Youssef N, Dagar SS, Fliegerova K, Griffith GW, Forster R, Tsang A, McAllister T, Elshahed MS 2014 Anaerobic Fungi (Phylum Neocallimastigomycota): Advances in understanding of their taxonomy, life cycle, ecology, role, and biotechnological potential. FEMS Microbiology Ecology 90 (1), 1-17
  • Puniya AK, Salem AZM, Kumar S, Dagar SS, Griffith GW, Puniya M, Ravella SR, Kumar N, Dhewa T, Kumar R 2014 Role of Live Microbial Feed Supplements with Reference to Anaerobic Fungi in Ruminant Productivity. Journal of Integrative Agriculture. Advance Online Publication Doi: 10.1016/S2095-3119(14)60837-6
  • Kumar S, Choudhury PK, Carro MD, Griffith GW, Dagar SS, Puniya M, Calabro S, Ravella SR, Dhewa T, Upadhyay RC, Sirohi SK, Kundu SS, Wanapat M, Puniya AK 2014 New aspects and strategies for methane mitigation from ruminants. Applied Microbiology and Biotechnology, 98, 31-44
  • Sirohi SK, Singh N, Dagar SS and Puniya AK 2012 Molecular tools for deciphering the microbial community structure and diversity in rumen ecosystem. Applied Microbiology and Biotechnology. 95 (5): 1135-1154
  • Kumar S, Dagar SS, Mohanty AK, Sirohi SK, Puniya M, Kuhad RC, Sangu KPS, Griffith GW & Puniya AK 2011 Enumeration of methanogens with a focus on fluorescence in situ hybridization. Naturwissenschaften, 98: 457-472
  • Sirohi, S.K., Pandey, N., Singh, B. and Puniya AK 2010 Rumen methanogens: a review. Indian Journal of Microbiology, 50: 253–262
  • Kumar S, Puniya AK, Puniya M, Dagar SS, Sirohi SK, Singh K, Griffith GW 2009 Factors affecting rumen methanogens and methane mitigation strategies. World Journal of Microbiology and Biotechnology, 25 (9), 1557-1566
  • Nagpal R, Yadav H, Puniya AK, Singh K, Jain S and Marotta F 2007 Potential of probiotics and prebiotics for synbiotic functional dairy foods: an overview. International Journal of Probiotics and Prebiotics, 2: 75-84
  • Nagpal R, Yadav H, Puniya AK, Singh K, Jain S and Marotta F 2007 Conjugated linoleic acid: sources, synthesis and potential health benefits - an overview. Current Topics in Nutraceutical Research, 5: 55-66
  • Goel G, Kumar A, Puniya AK, Chen W and Singh K 2005 Molecular beacon: A multitask probe. Journal of Applied Microbiology. 99: 435-442
  • Goel G, Puniya AK, Aguliar CN and Singh K 2005 Interaction of gut microflora with tannins in feeds. Naturwissenschaften, 92: 497-503
  • Kumar A, Goel G, Fehrenbach E, Puniya AK and Singh K 2005 Microarrays: the technology, analysis and application. Engineering in Life Science, 5: 215-222
  • Yadav H, Jain S, Puniya AK and Jain DK 2004 Internet resources of dairy: a mouse clicks away. Indian Journal of Dairy and Bioscience. 57: 135-144
  • James L, Puniya AK, Mishra V and Singh K 2002 Ozone: a potent disinfectant for application in food industry - an overview. Journal of Scientific and Industrial Research, 61: 504-509
  • Singh K, Puniya AK and Singh S. 1996 Biotransformation if crop-residues into animal feed by solid state fermentation. Journal of Scientific and Industrial Research, 55: 472-478
  • Puniya AK, Singh S, Ganeshkumar C and Singh K 1995 Single Cell Protein - A promising dietary substitute. Indian Journal of Experimental Biology, 33: 545-551
  • • Mahajan R, Chandel S, Puniya AK and Goel G 2020 Effect of pretreatments on cellulosic composition and morphology of pine needle for possible utilization as substrate for anaerobic digestion. Biomass and Bioenergy. https://doi.org/10.1016/j.biombioe.2020.105705
 
 
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