National Dairy Research Institute

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Home - Divisions - Dairy Microbiology
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Dairy Microbiology
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Faculty profile
keywordDairy Microbiology Division is currently engaged in research, teaching, consultancy, training and technology transfer in specialized field of Dairy Microbiology. Broadly, the research work of the division covers the areas related to starter cultures and fermented milk products; direct vat starters (DVS); indigenous probiotics, their functional efficacy and gut microbiota, prebiotics and synbiotics; bioactive peptides, microbial metabolites and biopreservatives; biosensors, quality assurance and food safety as well as rumen micro-organisms.  Division has played a leading role in establishing National Collection of Dairy Cultures (NCDC) with current repository of more than 600 cultures and National Referral Centre for milk quality and safety. The division has recently transferred technologies on two indigenous strains of probiotics, Misti Dahi, rapids kits for antibiotic residues, Listeria monocytogenes and Enterococci to potential stake holders in our country for their industrial application. Few more such products like carbonated lassi, vitamin B12 rich propioni-yoghurt, blueberry fortified probiotic dahi, real time test for detection of E. coli and antibiotic residues in milk have been developed and are under process of validation for their commercialization. The faculty is also involved in imparting teaching and guidance for both under-graduate and post-graduate programmes. The Division offers M. Tech and Ph.D programmes in Dairy Microbiology. Besides, division is also contributing in teaching Microbiology courses for B. Tech (Dairy Technology) students. The division offers contractual and consultancy / training services such as supply of starter cultures, freeze-drying of cultures, microbiological analysis and setting standards for regulatory compliance of dairy products in our country. Division regularly organizes need based specialized short term symposia/ conferences/ Seminars covering basic and applied areas of dairy microbiology including quality assurance, food safety, starter cultures and fermented health foods for HRD development in our country.
Research Laboratories/Units
Molecular Biology Unit (Probiotics and Gut Microbiota) : Dr. Sunita Grover and Ms. Rashmi, H.M. keyword
Molecular Biology Unit (MBU) was created during early 1990s as a centre for carrying out research and teaching activities related to Process Biotechnology. The unit has been established as a center of excellence with state of the art facility to pursue hard core science in frontier areas of molecular biology and dairy biotechnology. The unit has been recently renovated to give it a modular look. Apart from carrying out basic and applied research, the unit is also involved in imparting specialized training in modern molecular biology techniques such as molecular cloning, PCR/Real time PCR (RT-qPCR) techniques, identification of bacteria by molecular tools, tissue culture etc. The current focus of the unit is on development of indigenous probiotic strains of Indian gut origin for their application as bio-therapeutic agents by studying their functional efficacy through gene expression in appropriate tissue culture and animal models. The unit has also initiated a metagenomic study on the healthy human subjects recruited from north and north east Indian population with entirely different food habits to look at the metagenome of their respective commensal gut microbiota and preponderance of specific phylotypes under different dietary conditions.

Anaerobic Microbiology : Dr. S. K. Tomar, in place of Dr. A.K. Puniya (as he is on Deputation upto May, 2018)
The anaerobic laboratory works on the diversity of anaerobic Bifidobacterial spp. and obligatory rumen fungi. The laboratory also conducts research on mitigation of methane emissions from enteric fermentation, metagenomic analysis of reductive acetogens, biomarkers for taxonomy of rumen fungi, direct-fed microbials, etc. Besides, focus is also on the management of life style diseases (i.e. diabetes and obesity) by exploring the role of probiotics (lactobacilli and bifidobacteria), especially through digestive enzyme(s) inhibition, conjugated linoleic/ linolenic acids and/ or development of dairy based functional food(s).

Techno-functional Starter Laboratory (TFSL) : Drs. Sudhir Kumar Tomar; Surajit Mandal and Pradip V. Beharekeyword
The research area of TFSL is “bio-prospection of native lactic acid bacteria strains for development of techno-functional starters”. Currently, the laboratory is actively engaged in bio-prospecting of native strains for lactic acid bacteria (LAB), their characterization for production of functional bio-molecules (exo-polysaccharides, galacto-oligosaccharides, low calorie sugars, vitamins), proteomic studies of LAB, formulation of single and mixed defined strain starters for fermented dairy foods, stable and active ready to use functional starters as DVS, development of functional fermented dairy foods and cost effective bioprocesses for production of functional metabolites.

Microbial Biosensor, Food Safety and Quality Assurance Laboratory : Dr. Naresh Kumar and Mr. Raghu, H.V.
keywordOur research group is working in the area of food safety and successful efforts have been carried out in the development of sensors for antibiotics, β-lactam group, aflatoxin M1 in milk using bacterial spores as bio-recognition molecules. Similar approaches have also been  executed to detect L. monocytogenes, E. coli and Enterococci in milk employing markers enzymes. New know- how so generated through external funded projects (MoFPI, NAIP, Outreach) were protected through patents and licensed to stake holders in our country for industrial application. Apart from research, state of the art infrastructure facilities which include clean room, BSL-3 containment and testing equipments (EMCCD, SPR, D-Count, VIDAS, Charm system) have been  also established for execution of inter-disciplinary/ externally funded research projects.

Functional Fermented Foods and Bioactive Peptides: Dr. Shilpa Vijkeyword
This cell is actively engaged in the research and development of functional fermented foods and bio-processing of whey into value added products. Processes have been standardized for various fermented dairy and non dairy foods and whey based products. The technologies have been developed for biofunctional fruit yoghurts, soy based yoghurt, soy dahi and whey based functional probiotic beverages. The laboratory is also engaged in production of antimicrobial bioactive peptides from milk protein like whey and casein, and also from soy proteins and biopreservation of dairy products. Besides, the laboratory is also involved in the research project on the production of bioethanol from whey using stress tolerant yeasts.

Synbiotic Functional Foods: Dr. Chand Ram
keywordThe laboratory is involved in the development of synbiotic functional fermented dairy foods fortified with probiotic, prebiotic and plant bioactive components. The research laboratory has developed process technologies for manufacture of probiotic tulsi dahi for cholesterol reduction, synbiotic Misti Dahi and yoghurt with improved food safety and shelf life, blueberry fortified probiotic dahi for prevention of colon cancer, probiotic direct vat set (DVS/DVI) cultures for short set dahi. Currently, we are focusing on prevention/treatment of non alcoholic fatty liver diseases (NAFLD), bio-transformation of cholesterol for hypocholesterolemic effects, bioremediation of toxic metals, antibiotics and aflatoxins, control of biofilms in dairy niche, food safety and bio-preservation of dairy foods by natural plant bioactive components.
Microbial metabolites and bio-preservation: Mr. Diwas Pradhan
The cell is engaged in isolation of bacteriocinogenic lactic acid bacteria, production, purification, characterization of bacteriocins and their application in bio-preservation besides development of bacteriocins based antimicrobial packaging for enhancing the shelf life and safety. New initiatives are being undertaken to develop new food preservation bio-systems for enhanced shelf life and ensured safety of dairy products without any physical treatments as well as antimicrobial active and intelligent packaging strategies based on antimicrobial film allowing controlled release of micro and nano -encapsulated bacteriocins.

Central Facilities
National Collection of Dairy Cultures (NCDC) : Dr. Sudhir Kumar Tomar keyword
The National Collection of Dairy Cultures (NCDC) is a national facility being maintained at the Division of Dairy Microbiology since 1960. The NCDC stock includes strains of bacteria, fungi and yeasts. The main interest of the collection lies in the lactic acid bacteria from dairy sources. Besides individual strain cultures, the NCDC also holds mixed strain (traditional) and defined strain-formulated cultures for making different varieties of cheese and fermented milks. The NCDC caters to the need of dairy industry, research organizations as well as educational institutions. It also organizes specialized training programmes and workshops etc. to provide know-how on maintenance and preservation of cultures, propagation of dairy starter cultures and other related aspects. The NCDC is an Affiliate Member of the World Federation for Culture Collections and is also registered with the World Data Center on Microorganisms (registration number WDCM 775). It has also been listed in the World Directory of Culture Collections published by the WDCM, Japan.

Electron Microscopy Centre : Dr. Sudhir Kumar Tomar
The Electron Microscopy Centre of the Division has been established as a central facility for carrying out research in the advanced area of ultra structure of microorganisms and microstructure of dairy foods. The Electron Microscopy Center at NDRI is a core facility providing all levels of technical support and consultation in the area of light microscopy, electron microscopy, and image analysis. The centre is located at Room No.110, G- Floor, Dairy Microbiology Division. It provides microscopy and specimen preparation services for biological and food science research. In addition, the centre offers necessary training and access to all microscopes and ancillary equipment to faculty, staff, and students of different divisions of the NDRI as well as external users. This centre is operated on a cost-recovery basis and fees are accessed for consumables, equipment use, and staff support. The major instruments in the EM Centre include a Scanning Electron Microscope (EVO 18, Zeiss, U.K.), Transmission Electron Microscope (Jeol, JEM-1010, Japan). The ancillary equipment includes Ultramicrotome, Sputter coater and Critical point dryer.
Central Instrument Laboratory : Dr. Pradip V. Behare
Divisional central instrument laboratory is well equipped with various latest equipments viz. Thermocycler, Gel Documentation System, Elisa Plate Reader, Microphotography Microscope, Spectrophotometer, High Speed Centrifuge, Water Purification System, Ice Flecking Machine etc. This facility caters to the needs of research scholars and students of the division as well as  the Institute.

National Referral Centre on Milk Quality and Safety : Dr. Naresh Kumar and Mr. Raghu H.V. keyword
National referral centre on milk quality and safety (NRCMQS) with funding under NAIP project was establishment at NDRI in 2014 for addressing the scientific issues on milk safety in our country with the mandate to provide scientific solution in specialized field of milk safety and quality assurance; to carry out Risk Profile Work on Potential Contaminants in milk and milk products; development of milk safety standards, sampling plan and testing procedures for regulatory compliance; Rapid Biosensor Based Tools/Assay/Kits for field application and to conduct training on HRD development on milk quality. State of the art laboratory having equipments such as Biosafety cabinets, VIDAS multiple pathogen detection system, RT- PCR, Multimode plate reader, Centrifuge, Automatic media preparatory cum sterilizer, Biacore Surface Plasmon Resonance system, D count flowcytometer etc.
Ongoing Projects
Externally funded projects
  • Validating procedures for the measurement of heat resistant spoilage and pathogenic bacteria in milk, to provide a scientific and technical basis for future developments in the management of food safety in South Africa, India and Brazil (IBSA)
  • Joint India-UK collaboration project on Development and application of lactic acid bacteria as designer probiotics (BBSRC)
  • Joint India-UK collaboration project on TRansnational Industrial Biotechnology: UTilization of Extremophiles (TRIBUTE) (BBSRC)
  • Joint India- New Zealand collaboration project Development of Resilient Probiotic Foods Designed for the Indian Market Conditions – collaboration with Riddet University (DST)

  • Network Programme on Veterinary Type Culture Collection (VTCC) - Rumen Microbes (ICAR)
  • Status of antibiotic resistance among commercially available probiotic bacteria (ICMR)
  • Healthy Foods: Production of stable and Active Probiotics (MoFPI)
  • Diversity of exopolysaccharides produced by lactic acid bacteria – (DST)
  • Scheme on - Dairy Microbes- VTCC (ICAR)
  • Digestive enzymes (α-glucosidase and α-amylase) inhibitory probiotic lactobacilli for controlling postprandial hyperglycemia  (DBT)
  • Microbial diversity of industrial waste polluted sites of Punjab: Biogeochemical interactions for recycling of metals and degradation of pollutants (DBT)
  • Outreach Programme on “Monitoring of drug residues and environmental pollutants  (ICAR)
  • NAE project on “Development and evaluation of spore based assay for detection of pesticide residues in milk (ICAR)
  • Screening probiotics and prebiotics for expression of Glucagon like peptide-1 (GLP-1), a satiety inducing hormone, as prophylactics against Diabetes Mellitus – 2 (ICMR)
  • Comparative metagenome of human gut of North and North-eastern region of India– in collaboration with Tezpur University (DBT)
  • Study of mechanism of probiotic action in persistent diarrhea in children caused by enter aggregative E. coli – using a mouse model – in collaboration with NICED (ICMR)
  • Whey to Biofuel: Bioethanol production by stress tolerant and metabolically engineered yeast from whey by ICAR (National Fund)
  • Microbial diversity of bio-films in dairy niche (AMAAS)

Important Research Findings
  • Whole genome Sequence of three indigenous probiotic Lactobacillus strains has been deciphered and annotated using Illumina platform
  • MLST scheme developed for differentiation of L. plantarum species at strain level could be a remarkable and valuable tool which can be used as a molecular identity card
  • The indigenous probiotics strains and their postbiotic preparations stimulated GLP-1 secretion and proglucagon expression from both human and mouse enteroendocrine cell lines.
  • The biofunctional properties of casein hydrolysates, whey protein hydrolysates and fermented milk protein hydrolysates in the secretion and expression of gut hormones have been unveiled.
  • Optimized process parameters for production of bioactive peptides from casein and whey proteins using Lactobacillus cultures that act as antifungal substances for preservation of paneer.
  • Developed blueberry fortified probioitc dahi for prevention of colon cancer, low cholesterol probiotic butter.
  • Developed soy and whey based fermented foods viz., yoghurt, dahi, fermented beverage, acido-whey and strawberry probiotic whey drink.
  • Process and production conditions for Vitamin B12 production by probiotic propionibacteria have been optimized.
  • Isolated and characterized Native Probiotic strains i.e. Lactobacillus rhamnosus RSI-3  with anti-diabetic and Lactobacillus fermentum RS-2 (isolated from indigenous cereal based fermented milk product) with hypoglycemic, Antimicrobial, immunomodulatory and hypo-cholesterolaemic attributes
  • Bio-production technologies for mannitol and trehalose from whey have been optimized
  • Production of Galactooligosaccharides (GOS) using β-galactosidase from native strains of Streptococcus thermophilus and  Kluyveromycesspp
  • Technology of preparation of vitamin B12 rich Propioni Yoghurt and reduced calorie naturally carbonated sweetened fermented dairy beverage.
  • Development of cereal based functional fermented milk product with extended shelf-life
  • Promising strains of probiotic lactobacilli with strong probiotic attributes and colonization potential have been characterized
  • A bacteriocin based food grade bio-preservative for bio-preservation of dairy foods and bacteriocin based formulations against mastitis has been developed.
  • Developed direct-fed rumen fungal inoculants for Methane mitigation from ruminants
  • Developed cost effective spore and enzyme based farm level test kit for detection of antibiotic residues in milk. Developed Iodometric based analytical process for specific detection of β-lactam group of antibiotics in milk.
  • Two stage enzyme assay for detection of Listeria monocytogenes in milk and milk products. Rapid enzyme/ spore based assay on 96 micro-well plate assay and biochip for detection of E. coli L. monocytogenes, Enterococci in milk developed.
  • Rapid enzyme/ spore based assay on 96 micro-well plate assay & biochip for monitoring Aflatoxin M1 in milk.
  • Biosensors and micro-techniques has been developed for detection of pesticide residues, aflatoxin, heavy metals and bacterial contaminants in milk
  • Anaerobic fungi Oontomyces anksri gen. nov., sp. nov isolated from the forestomach of an Indian camel (Camelus dromedarius L.)
  • Characterization of new CLA (c9, t11, t10, c12, t9, t11) producing Lactobacillus strains from infant stools

International collaboration
  • University of Pretoria, South Africa.
  • Pucars, Brazil
  • BBSRC - Biotechnology and Biological Sciences Research council, UK
  • Riddet Institute University Ave, Massey University, Palmerston North 4474, New Zealand
  • Tezpur University
  • National Institute of Cholera and Enteric Diseases (NICED)
  • CSRI-National Chemical Laboratory (NCL)
  • University of Delhi
  • TERI - The Energy and Resources Institute
  • CSRI-Central Food Technology Research Institute (CFTRI)
  • Indian Veterinary Research Institute (IVRI)
  • National Research Centre (NRC) on Yak
  • National Institute of Animal Nutrition and Physiology (NIANP)
  • National Research Centre on Equines
  • State agriculture Universities (SAU’s)
  • Sikkim University, Gangtok
  • National Bureau of Agriculturally Important Microorganisms, Mau
  • Guru Nanak Dev University, Amritsar
  • Central institute of Post Harvest Engineering And Technology (CIPHET), Ludhiana
Technologies and Patents
Technology Transferred
  • Technologies on two indigenous probiotic strains of Lactobacilli were transferred to M/s Sarvotham Care Ltd., Secunderabad through MoU exchange at ICAR, Krishi bhawan on 30th Sep 2014. A step towards “Make in India”
  • A Technology for production Misti Dahi having improved body and texture using well characterized fast acidifying high sugar tolerating lactic culture was transferred to Namaste India Foods Pvt. Ltd., Kanpur-U.P through MOU exchange on 1stNovember 2014.
  • Technology on “Detection of Enterococci in milk” was also transferred to M/s Neugen Diagnostics (India) Pvt. Ltd., Hyderabad as non-exclusive license with lumsump price of Rs 3.5 lakhs through MOU (ref. no. 11146 ) exchange vide dated 5/07/2013 during Agri-Tech Meet organized at NASC complex dated 18-19th July 2013.
  • Technology on “ Detection of Listeria monocytogenes in milk” was transferred to M/s Neugen Diagnostics (India) Pvt. Ltd., Hyderabad as non-exclusive license with lumsump price of Rs 7.5 lakhs through MOU (ref. no. 11147 ) exchange vide dated 5/07/2013 during Agri-Tech Meet organized at NASC complex dated 18-19th July 2013

Technologies Developed
  • Enzyme-Spore based assay for detection of antibiotic residues in milk
  • Enzyme-Spore based assay for detection of pesticide residues in milk
  • Curcumin Soy whey drink
  • Strawberry whey drink
  • Health promoting soy yoghurt
  • Lactose free soy dahi
  • Probiotic drink with antidiarrheal activity
  • Development of EPS producing low fat lassi
  • Bioprocess for Direct Product Probiotic (DPP) formulation of Lactobacillus culture
  • Direct Vat Set (DVS) Misti Dahi Culture
  • Blueberry fortified probiotic Dahi for prevention of colon cancer
Patents Granted/ Filed
  • A Novel Process of Sporulation, Activation and Germination in Thermophilic Bacteria for Rapid Detection of Antibiotic Residues in Milk. Kumar N., Patil G. R., Rane S., Malik R. K. (2014). Indian Patent No. 264145 dated 9.12.2014. (Granted)
  • Naresh Kumar, Alia Khan, Falguni Patra, Menakshi Dahiya, Shivani Arora, Mandeep Balhara, Pradeep Kumar Sharma, Raghu H.V. and Suleman Shaikh (2014). Patent entitled “Development of Enzyme-Spore based assay (s) for monitoring antibiotic residues in milk” Patent: Reg # IPR 2213/DEL/2014.
  • Naresh Kumar, Ramakant Lawaniya, Avinash, Bhawna Arora, Raghu H.V., Mandeep Balhara, Saurabh Kadyan, Vinai Kumar Singh (2014). “Spore-sensor for rapid detection of E. coli in milk employing marker enzymes and marker sugar as germinants”. Patent: Reg # IPR 2214/DEL/2014.
  • Balhara, M., Kumar, N., Thakur, G., Raghu, H. V., Singh, V. K., Lawania, R., Khan, A., Shabnam(2013). Novel enzyme-substrate based bio-assay for real time detection of Listeria monocytogenes in milk system. Patent Reg # IPR# 1357/DEL/2013.
  • Kumar N, Kaur G, Thakur G, Raghu HV, Singh N, Singh VK and Raghav N (2012). Real time detection of Enterococci in dairy foods using spore germination based bioassay. Patent Reg # IPR 119/DEL/2012.
  • Kumar, N., Singh, N.A., Singh, V.K., Bhand, S. and Malik, R. K.  (2010). Development of a real time process for monitoring Aflatoxin M1 in milk using spore inhibition based-enzyme substrate assay (SIB-ESA). Patent Reg # IPR 3064/DEL/2010-Published in Indian patent Journal No. - 46/2012 dated 16/11/2012.
  • Kumar N., Das S. and Gaare M. (2010). Development of Iodometric based lab-scale kit for specific detection of β-lactam group of antibiotics residues in milk. Patent: Reg # IPR 115/DEL/2009- Published in IPO journal –U/S 11A dated 30/7/2010.

Awards and Recognitions
  • Ms Rashmi, H. M., Scientist, Molecular Biology Unit, Dairy Microbiology Division,  was conferred Young Investigator Award (First award of Rs. 10,000) at PAi conference (2014)
  • Fellow of National Academy of Dairy Sciences, India, 2014
    • Dr. Naresh Kumar
    • Dr. A.K. Puniya
  • Fellow of ANSI; Animal Society of Nutrition, India; 2014 (A. K. Puniya)
  • Advisory Committee member; ICBB-2014, Dubai, UAE (A.K. Puniya)
  • Member of scientific Panel member for Biological hazards by the Food Safety and Standard authority (FSSAI) of INDIA (Naresh Kumar)
  • Appreciation from “World Bank team members” (2014) during their visit at NDRI for evaluating the performance of National Referral Center for Milk Quality and Safety established with fund provision (17.5 crores) from NAIP (Naresh Kumar)
  • Best division award 2012-13 by ICAR-NDRI, Karnal (R.K.Malik)
  • European Union ERASMUS MUNDUS visiting Fellow  Award (R.K.Malik)
  • N N Dastur Award 2011 by NDRI Almuni Association (Dr. R.K. Malik)
  • Young Women Bio-scientist Award by Department of Biotechnology Govt. of India (Sunita Grover)
  • INSA-Royal Society Fellowship by Indian National Science Academy (A.K. Puniya)
  • Kautilya Gold Medal (A.K. Puniya)
  • Bharat Excellence Award and  Gold Medal by Friendship Forum of India, Delhi (Chand Ram)
  • Dr. K.K. Iya Award by ICAR- National Dairy Research Institute, Karnal (A.K. Puniya)
  • Prof. J.V. Bhat Memorial Award by Association of Microbiologist of India (A.K. Puniya)
  • Indian Delegation Nominee for codex meeting on drug residues, Codex Alimentarius, Minneapolis, Minnesota.(Naresh Kumar)
  • Honorable Mention’, American Society for Microbiology ‘Moselio Schaechter Distinguished Service Travel Award’, 2010 (A.K. Puniya)
  • Young Scientist Award, Environmental Microbiology, Association of Microbiologists of India, 1996 (A.K. Puniya)
  • DBT-CREST’ Award (2011-12), to visit UK (A.K. Puniya)
  • German Academic Exchange Service (DAAD) Fellowship, 1992 (A.K. Puniya)
  • Member ‘International Scientific Advisory Board’, BioMicroWorld2015, Conference of Formatex Research Centre, Spain, (A.K. Puniya)
  • Certificate of Appreciation,   The Annual Bio-medical Research Conference for Minority Students’, USA,  2014 (A.K. Puniya)
  • Jawaharlal Nehru Award for P.G. Outstanding Doctoral Thesis Research in Agricultural and Allied Sciences 2010 (Dr. Ramya Iyer; Guide : Dr. S. K. Tomar)
  • Jawaharlal Nehru Award for P.G. Outstanding Doctoral Thesis Research in Agricultural and Allied Sciences 2011 (Dr. Raj K Duary; Guide : Dr. Sunita Grover)
  • Jawaharlal Nehru Award for P.G. Outstanding Doctoral Thesis Research in Agricultural and Allied Sciences 2013 (Dr. Gurpreet Kaur; Guide: Dr. R.K. Malik)
  • Best M. Tech thesis award 2009 by NDRI Deemed University (Ms Archana Chandran; Guide : Dr. V.K.Batish)
  • Best Ph.D. thesis award 2009 by NDRI Deemed University (Dr. Raj K Duary; Guide: Dr. Sunita Grover)
  • Best young researcher award (second prize) awarded to Dr. Raj Kumar Duary (Guide: Dr. Sunita Grover) by Probiotic Association of India (PAI)
  • Best Ph.D. thesis award 2009 by NDRI Deemed University (Dr. Ramya Iyer; Guide : Dr. S. K. Tomar)
  • Best Ph.D. thesis award 2012 by NDRI Deemed University (Dr. Gurpreet Kaur; Guide: Dr. R.K. Malik)
  • Best Ph.D. thesis award 2013 by NDRI Deemed University (Dr. Santosh Kumar Mishra; Guide: Dr. R.K. Malik)
  • Best Ph.D. thesis award 2014 by NDRI Deemed University (Dr. Kiran Thakur; Guide: Dr. .S. K. Tomar)

Teaching Activities

DM 711: Microbial Diversity and Physiology (3+0)
DM 712: Advances in Microbial Genetics (3+0)
DM 721: Advances in Dairy and Food Microbiology (3+0)
DM 722: Advances in Microbial Safety of Dairy Foods (3+0)
DM 719: Credit Seminar-I (0+1)
DM 729: Credit Seminar-II (0+1)

M.Tech/ M.V.Sc./ M.Sc

DM 611: Microbial Morphology and Taxonomy (2+1)
DM 612: Microbial Physiology (2+1)
DM 613: Microbiology of Fluid Milk and Dairy Products (2+1)
DM 614: Environmental Microbiology (2+1)
DM 615: Microbiology of Processed Dairy Foods (2+1)
DM 621: Starter Cultures and Fermented Dairy Products (2+1)
DM 622: Microbial Genetics (2+1)
DM 623: Microbiological Quality Assurance in Dairy Industry (2+1)
DM 624: Probiotics for Health Foods (2+1)
DM 625: Research Techniques in Dairy Microbiology (2+1)
DM 626: Microbial Fermentation Technology (2+1)
DM 629: Credit Seminar (0+1)


DM 111: Fundamentals of Microbiology (2+1)
DM 122: Introductory Dairy Microbiology (2+1)
DM 311:Starter Culture and Fermented Milk Products (2+1)
DM 312: Quality and Safety Monitoring in Dairy Industry (2+1)
DM 323: Food and Industrial Microbiology (2+1)
3M Petrifilm Reader, Automated Media Preparatory Cum-Sterilizer-Dispenser, Bench Top Fermenter, Bench Top Refrigerated Incubator Shaker, Block Heater, BOD Incubator , Biosafety Cabinets - (BSL I, II &III), Biosafety Cabinets  (A2 type), Biosafety Cabinets (B2 type), Carbon Dioxide Incubator, Charm Rosa Lateral flow Assay System, Charm System, Cold Room, D-Count Flow Cytometer (System for enumeration of hygiene indicators), Data Logging System (LIMS), Deep Freezer (– 200C, - 80°C), DGGE, Electron Multiplying Charged Coupled Device (EMCCD), Electroporator, ELISA Plate Reader, EMCCD Camera, Freeze Drier, Gel Documentation Systems, Gel electrophoresis unit, High Speed refrigerated centrifuge (with 500 ml, 50 ml, 30 ml and 1.5 ml rotors, HPLC, Ice Matic Flaker/Ice Making Machine, Incubator Shaker, Lab Scale Spray Dryer, Liquid Handling System, Microbiological Plate Reader, Liquid Handling System, Mass Spectrometer Facility, Microccentrifuge, Microfiltration Unit, Microfuges, Microprocessor Controlled refrigerated incubator shaker, Microscopes:  (Inverted, Phase contrast, Scanning & Transmission Electron), Microfiltration System, Modular Cold Room, Multimode Plate Reader –Auto-Analyzer for Detection of Total Aflatoxin, PCR Thermal Cycler, Petrifilm Reader, Plate Reader, Real Time PCR for Pathogen Detection, Real Time PCR Machine, Refrigerated Centrifuge, Refrigerated Incubator Shaker, ROSA Reader Alongwith Incubators, Rotational Vacuum Concentrator System, Sonicator (Ultrasonic Processor), Spectrophotometer, Surface Plasmon Resonance (SPR) Biacore-3000- High Throughput Real-Time system, Speed Vac System, Tecan 200 M Pro Multimode Plate Reader, Table Top Concentrator, UV Spectrophotometer, UV transilluminator, Ultra Low Deep Freezer (-85°C), Ultracentrifuge  Ultramicrotome, Sputter Coater and Critical Point Dryer, VIDAS (Multiple Pathogen Detection System), Walk in Cold Room, Water Purification System
Consultancy service
The Division provides consultancy services on different aspects to the dairy and food industries.
  • Starter cultures for dairy industry
  • Microbiological Quality testing
  • Probiotic cultures
  • Microstructural analysis
  • Establishment of Pathogen testing lab.
  • Synbiotic functional foods

The following technologies were validated:
  • Probiotic counts of Amul Pro Life, a trend setter First probiotic dairy foods launched by Amul, Anand In India
  • Probiotic attributes of Nesvita Dahi, a certificate to this effect to be used by Nestle India on the cover of package itself
  • Preparation and analysis of Dahi” carried out for M/S. Samsung India Electronics Private Limited, South Korea
  • Identification and safety evaluation of NCDC 428 culture for commercial production of Misti Dahi

Faculty profile
Dr. Sunita Grover Principal Scientist & Head
Dr. A.K. Puniya Principal Scientist (on Deputation w.e.f. 28.05.2015 for 3 Years)
Dr. S.K. Tomar Principal Scientist
Dr. Shilpa Vij Principal Scientist
Dr. Naresh Kumar Principal Scientist
Dr. Chand Ram Principal Scientist
Dr. Surajit Mandal Scientist (Senior Scale)
Dr. Raghu H. V Scientist
Dr. Pradip. V. Behare Scientist
Dr. Rashmi H. M Scientist
Dr. Diwas Pradhan Scientist

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