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Home- Divisions - Animal Biotechnology Centre
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Animal Biotechnology Centre
Current Research Projects at ABTC Courses Offered
Achievements Technologies Patented
Transfer of Technology Current area of Research
Faculty Profile

Biotechnology was initiated at NDRI, Karnal during mid eighties under a UNDP 'Centre of Excellence on Biotechnology' program. The urgent need for application of recent biotechnological advances in reproduction and production to superior females of dairy breeds of ruminants for improving animal productivity in our country formed the basis for the establishment of a state-of-art Embryo Biotechnology Centre (EBC) by financial support from the Department of Biotechnology. Biotechnology was further strengthened by establishment of Livestock Genome Lab and Molecular Biology Unit under National Agricultural Research Project-II. Animal Biotechnology Centre was reorganized in June 1999 by consolidating all the infrastructure facilities created under various programs on biotechnology. Besides research on areas relevant to biotechnology in dairy production and processing, the Centre also offers M.Sc./M.V.Sc./M.Tech and Ph.D. (Animal Biotechnology) programmes.

The objectives of the proposed Division are 1) To undertake biotechnology oriented basic and applied research programmes for improving animal productivity and for developing innovative dairy processes for producing superior quality, safe and wholesome dairy products, 2) To train manpower in application of Biotechnology in Dairy Production and Dairy Processing and 3) To organize M.Sc. and Ph.D. programmes in Biotechnology for the NDRI Deemed University.

The three main labs at the Animal Biotechnology Centre are Embryo Biotechnology Lab, Animal Genomics Lab and Proteomics Lab. A state-of-the-art biotechnology research facility was created in 2007 to consolidate the biotechnology research at NDRI. The new lab facility offers a working space of more than 20,000 sq. ft. and has specialized laboratories on Embryo Biotechnology, Regenerative Biotechnology, Animal Genomics, Proteomics Research, Structural Biology etc.   


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Current Research Projects at ABTC

Leader Dr. P.Palta

·       Productionof multiple copies of elite buffalo bulls using animal cloning technology.(31.03.2018 to 30.03.2022). Funded by NASF (Rs. 264.29 lakhs).

·       Globaltranscriptome and miRNA analysis for deciphering reasons for low cloningefficiency in buffalo. (22.03.18 to 21.03.2021). Funded by SERB-DST (Rs. 46.78lakhs).

Leader Dr. T.K.Datta

·       MolecularMarkers for Improving Reproduction of Cattle and Buffaloes. (31.07.2018 to31.07.2023). Funded by NASF (Rs. 648.38 lakhs). Leader Dr. T. K. Datta.

·       Improvingthe usability of buffalo spermatozoa by sperm surface remodelling and immuneacceptance in female reproductive tract. (01.07.2018 to 30.07.2021). Funded byNASF (Rs. 174.60 lakhs). Leader Dr. T. K. Datta.

·       Decipheringthe circulating miRNAs from terminal stage pre-implantation embryos andplacentomes for early detection of pregnancy in buffalo. (04.08.2016 to03.07.2019). Funded by DBT (Rs. 52.27 lakhs). Leader Dr. T. K. Datta.

Leader Dr. S. De

·       Geneticvariability of milk protein and its characterization by proteomic approach inIndian goats. (01.06.2017 to 31.05.2020). Funded by NASF (Rs. 34.45 lakhs).

·      Mastitis-related bacterialantibiotic resistance pattern mapping in Karnal district. (1.4.2017to 31.3.2020). Institute project.

Leader Dr. J.K.Kaushik

·       Elucidating the mechanism of pashmina fibre development: AnOMICS approach (01.07.2015 to 31.12.2018). Funded by NASF (Rs. 131.35lakhs).

Leader Dr. A.K. Mohanty

·       DelineatingBeta Casein Variants in Indian Cows and Potential Health Implications of A1A2Milk. (01.07.2015 to 31.12.2018). Funded by NASF (Rs. 58.55 lakhs).

·       Synthesis,characterization and effect of graded levels of nano-selenium supplementation onthe performance of broiler chicken. (01.06.2017 to 31.05.2020). Funded by NASF(Rs. 42.72 lakhs).

·       Genomeediting of MFGE8 and S100 genes in bovine mammary epithelial cells tounderstand their role in milk production. (21.03.2018 to 20.03.2021). Funded bySERB-DST (Rs. 62.53 lakhs).

·       Semensexing in cattle. (01.04.2017 to 31.03.2020). Funded by ICAR (Rs. 239.50lakhs).

·       Developmentof early pregnancy diagnostic assay through discovery of biomarkers in cattleand buffalo. (10.05.2018to 09.05.2021).Funded by DBT (Rs. 114.70 lakhs).


Leader Dr. D.Malakar

·       CRISPR/CAS9guided functional analysis of genes regulating early embryonic survival inbuffalo. (01.08.2018to 31.07.2021).Funded by NASF (Rs. 62.44 lakhs).

·      Use of mesenchymal stem cells for treatment of mastitis and metritis inbovine. (1.4.2018to-31.3.2020). Institute project.

Leader Dr. SatishKumar

·       Production of CRISPR-Cas9 mediated β-lactoglobulin gene-editedbuffalo embryos.  (1.4.2018 to31.3.2021). Institute project.

Leader Dr. RakeshKumar

·       Targetedimmobilization of Y-bearing spermatozoa and modulation of oviduct milieu forskewing sex ratio towards female offspring in dairy cattle. (01.08.2018 to31.07.2021). Funded by NASF (Rs. 11.34 lakhs).

·      Characterization of beta-defensin genesand their relation with bull fertility. (1.4.2017 to 31.3.2020). Instituteproject.

Leader Dr. ManojKumar

·       Conservationof Indigenous Pig of Assam through Handmade Cloning Technique. (02.01.2017 to01.01.2020). Funded by DBT (Rs. 40.59 lakhs).

Leader Dr. SudarshanKumar

·       Leukemiainhibitory factor: pluripotency in buffalo stem cells. (01.07.2015 to 30.06.2018). Funded byNASF (Rs. 126.51 lakhs).

Identification and targeted validation of unique proteins expressed duringsubclinical mastitis in Sahiwal and Karan Fries cattle. (1.4.2016 to 31.3.2019). Institute project.

IRC projects

  • Identification of stage-specific small, non coding RNAs in buffalo oocytes and pre-implantation embryos. Leader Dr. Rakesh Kumar
  • Production of buffalo recombinant interferon-tau and evaluation of its biological activity. Leader Dr. Malakar
  • Generation of induced pluripotent stem (IPS) like cells from buffalo fibroblasts. Leader Dr. M.K. Singh
Externally funded projects
  • Deciphering the mechanism of aberrant maternal recognition of pregnancy (MRP) events in sheep and buffalo under heat and nutritional stress.  (NASF) Leader Dr. T.K. Datta
  • Genetic diversity and haplotyping of innate immune genes in Indian cattle and buffalo (DBT) Leader Dr. S. De
  • Anti-Mullerian hormone profile, its characterization and expression for using as diagnostic marker for fertility assessment in farm animals (DBT) Leader Dr. S. De
  • Early detection of pregnancy in cow and buffalo by pregnancy associated proteins (PAPs) (NASF) Leader Dr. A.K. Mohanty
  • Development of transgenic goat for production of human lactoferrin- (NASF). Leader Dr. M.S. Chauhan
  • Enhancing development competence of oocytes for better in vitro fertilizing ability (NASF). Leader Dr. T.K. Datta
  • Development of parthenogenetic goat from embryonic stem cells (NASF) Leader Dr. M.K. Singh
  • Countering Gastrointestinal Tract Pathogens by Adhesion-Promoting Probiotic Surface Proteins (NASF) Leader Dr. J. K Kaushik
  • Recombinant expression of lactobacilli aminopeptidases for the production of bioactive peptides. (DBT) Leader Dr. J.K. Kaushik
  • Cloning of wild buffalo in Udanti-Sitanadi of Chhattisgarh. (Govt. of Chhattisgarh) Leader Dr. S.K. Singla
  • Optimization of procedures for improving cloning efficiency in buffalo. (DBT) Leader Dr. S.K. Singla
  • Buffalo transgenesis for expression of desired proteins. (DBT). Leader Dr. M.S. Chauhan
Courses Offered
BT 611 Fundamentals of cell & molecular biology
BT 612 Animal cell culture: principles & applications
BT 613 Immunology applied to biotechnology
BT 614 Reproductive biotechnology
BT 621 Applied molecular biology
BT 622 Molecular diagnostics
BT 623 Animal genomics
BT 624 Techniques in molecular biology & genetic Engineering
BT 629 Master’s Seminar
BT 639 Master’s Research
BT 711 Gene cloning and expression
BT 712 Functional genomics & proteomics
BT 713 Advances in reproductive biotechnology
BT 719 Doctoral seminar  
BT 721 Trends in Vaccinology
BT 722 Advances in animal cell culture
BT 729 Doctoral Seminar II
BT 739 Doctoral Research

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  • A female calf named ‘Deepasha’, which was a clone of a wild buffalo named ‘Asha’, that has been kept in semi-captivity at Chhattisgarh, was born on December 12, 2014 by normal parturition. Wild buffalo is an endangered species.
  • A male cloned calf named ‘Rajat’, produced through ‘Hand-guided Cloning’ was born on July 23, 2014 by normal parturition. Rajat is a clone of a highly ranked progeny-tested Murrah buffalo bull (MU-4393) which had died many years back.
  • A female cloned calf named ‘Lalima’, produced through ‘Hand-guided Cloning, was born on May 2, 2014 by normal parturition. Lalima, which is now 7 months of age, is a clone of an elite Murrah buffalo (MU-5345) of NDRI Livestock Farm.
  • Many significant achievements of Animal Biotechnology Centre, including those considered as world records (WR), were highlighted in the Limca Book of Records, 2014.
  • Achievements of ABTC in Limca Book of Records, 2014
  • Cloned buffalo embryos were successfully produced using trophoblast cells, urine- and milk-derived somatic cells and lymphocytes as donor cells.
  • Cloned blastocysts derived from fibroblasts, milk-derived cells, lymphocytes, trophoblast cells and those produced by in vitro fertilization were found to differ in their developmental competence, level of apoptosis, epigenetic status and expression level of many important genes.
  • Buffalo trophoblast cell lines were developed from blastocysts produced by in vitro fertilization, Hand-guided cloning and parthenogenesis. A feeder-free in vitro culture system was developed which enabled their long-term culture.
  • It was demonstrated that staining with brilliant cresyl blue can be effectively used for selection of developmentally competent oocytes for Hand-guided cloning.
  • Treatment of buffalo donor cells with epigenetic modifiers was shown to improved the blastocyst rate and quality of cloned embryos
  • Cloned female and male buffalo embryos were found to differ in their developmental competence, epigenetic status, sex-biased transcription patterns in X-linked genes and response to epigenetic modifiers. Aberrant X-linked gene expression occurred frequently in female embryos.
  • Lowering the oxygen tension during IVC of buffalo embryos from 20 to 5% was found to improve their developmental competence and quality, and reduce the apoptosis level.
  • Transgenic cloned buffalo embryos were produced containing pAcGFP-buSCD, a mammary gland specific expression vector, which is able to convert high level of conjugated linoleic acid (CLA) in mammary gland epithelial cells.
  • The period of oocyte maturation was found most vulnerable to be affected by heat stress in buffalo. Elevated temperature exposures during this time were found to result in compromised embryo developmental rate. A suitable intervention during the oocyte maturation (pre-ovulatory phase) may prove useful in augmenting fertility overcoming the environmental stress.
  • Functional characterization of Milk fat globule membrane epidermal growth factor 8 (MFGE8) was performed by gene silencing using shRNA. It was observed that MFGE8 is essential for maintenance of cell shape and morphology during lactation in farm animals.
  • Various extraction methods were optimized for extraction of proteins from bovine urine.  1600 proteins were identified in bovine urine by LC-MS/MS for the first time.
  • Three potential urine protein biomarkers (bovine allergen, Mannan-binding lectin serine peptidase 2, Alpha-1-microglobulin, Glutaminyl-peptide cyclo-transferase) were identified for detection of early pregnancy (~ 20 days) in cows. Three proteins namely vitamin-binding protein, apolipoprptein A and complement 3 were up-regulated in bovine serum during pregnancy (16th to 45th day).  
  • Buffalo recombinant pregnancy-associated glycoprotein PAG1 and PAG2 were expressed in E. coli.
  • Differential transcript analysis in fetal cotyledon of early pregnant buffalo (~2 months) revealed that prostate-specific antigen (PSA) is over expressed during early pregnancy.
  • Recombinant mucus binding protein (Mub) and S-layer protein (Slp) of lactic acid bacteria were expressed in biologically active form. These proteins showed binding with human gut tissue and gut cell lines.

In last one year two patent have been granted for following two technologies.

  • A non-invasive DNA isolation methodology in Dairy Animals.
  • A PCR based method for differentiating Cow and Buffalo milk

Two technologies from Animal Biotechnology Centre were transferred to A2 Milk Research Corporation, Raipur and a MOU has signed on 1st December 2014. These are
i)         PCR-based method for differentiating Cow and Buffalo milk and
ii)        PCR-based method for differentiating A1 and A2 milk.


Embryo Biotechnology
  • Embryonic stem cell technology
  • Animal cloning
  • Transgenesis
  • Sperm sexing
Animal genomics
  • Oocyte/embryo genomics
  • Gene polymorphism
  • Genomics of male fertility
  • Genomics of immunity
Proteomics and structural biology
  • Early pregnancy biomarker
  • Proteomics of milk production and lactation
  • Probiotics surface proteins
  • Sperm proteomics
  • Bioinformatics
Faculty profile
Dr. Prabhat Palta Principal Scientist, In-charge
Dr. T.K.Datta Principal Scientist
Dr. S De Principal Scientist
Dr. D.Malakar Principal Scientist
Dr. A.K.Mohanty Principal Scientist
Dr. J.K. Kaushik Principal Scientist
Dr. Rajesh Kumar Senior Scientist
Dr. Rakesh Kumar  Principal Scientist
Dr. Satish Kumar Principal Scientist
Dr. Manoj Kumar Singh Scientist
Dr. Sudarshan Kumar Scientist
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